Composite

Part:BBa_K2790006:Design

Designed by: Shrestha Rath   Group: iGEM18_IISER-Bhopal-India   (2018-10-10)


smmoD gene with a eukaryotic promoter reporter construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 115


Design Notes

The gene and pAOX-iLOV construct was put together using 3A Assembly


Source

The smmoD gene (BBa_K1390003) construct obtained from the Registry was flanked with the constitutive eukaryotic promoter (GAP) via traditional cloning. The AOX promoter- iLOV construct was synthesised as a gBlock from IDT.

References